Part:BBa_J435116

pOSIP-TT (TetR, P21)

One-Step Cloning and Chromosomal Integration of DNA

Contains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.

https://freegenes.github.io/genes/BBF10K_000493.html

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal suffix found in sequence at 1125
Illegal EcoRI site found at 1
Illegal XbaI site found at 28
Illegal XbaI site found at 1397
Illegal XbaI site found at 5490
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal NheI site found at 6090
Illegal SpeI site found at 1126
Illegal PstI site found at 1140
Illegal NotI site found at 1133
Illegal NotI site found at 5025
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
Illegal BglII site found at 469
Illegal BamHI site found at 22
Illegal XhoI site found at 1152
23
INCOMPATIBLE WITH RFC[23]
Illegal suffix found in sequence at 1126
Illegal EcoRI site found at 1
Illegal XbaI site found at 28
Illegal XbaI site found at 1397
Illegal XbaI site found at 5490
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal XbaI site found at 28
Illegal XbaI site found at 1397
Illegal XbaI site found at 5490
Illegal SpeI site found at 1126
Illegal PstI site found at 1140
Illegal AgeI site found at 1343
Illegal AgeI site found at 3052
Illegal AgeI site found at 4681
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 357
Illegal BsaI.rc site found at 3197